Call 859-261-2035. When you release, the tagged ear will fall out of the applier. Again, clean the punch with a disinfectant, rinse with alcohol and dry it thoroughly to prevent rusting. Next, in order to quantify cancer progression, they imaged the animals using bioluminescence at one, ten and eighteen days post injection. Because the implants are relatively large, they are not recommended for newborns, but they are acceptable for identifying weanlings and adults. WEB Administration Of Drugs and Experimental Compounds in Mice and Rats Updated January 1, 2014 PDF Animal Care Consent to Treat Updated July 26, 2016 PDF Animal Care Med Campus Animal Order Form Updated May 21, 2020 This protocol measures the extent to which individual mice discount the value of delayed food. Most mice will defecate within a minute of being picked up. At this time, the animal should be identified by an appropriate method (ear tag, transponder, ear punch,…) 6 One disadvantage of metal tags is that it is often necessary to pick up the animal in order to read the code. IACUC protocol. ... Small amounts of DNA can be obtained from ear punches, tail biopsies, or various non- Several systems are available for individually identifying research mice. Ear tagging: A metal tag with a unique identification number is attached to one ear of the rodent. Used to deliver personalized information and tailor communications. The tattoo is placed on a toe or tail. Ear tagging. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. • Gently restrain mouse with gloved hands by scruffing the dorsum high on the neck to control both the body and the head. The use of toe tattooing allows such animals to be identified until they are large enough for ear tagging or ear punching. Slip the tag out so that the crimped end does not pass through causing trauma and enlargement of the piercing hole. These include: metal tags that have a series of numbers and/or letters etched on them, and plastic tags with characters or barcodes on brightly colored backgrounds. Thank you for taking us up on our offer of free access to JoVE Education until June 15th. However, ear tags are more difficult to read than tattoos and can fall out, and mice usually require restraint for … Unable to load video. Ear Tags Ear tags are another means for identifying mice. In order to remove the tag, moisten a cotton-tipped applicator with an antiseptic solution and use it to remove any debris from around the tag. Using hemostats, gently guide the ear tag through and out of the ear. Depending on the position, notches on the right ear represent single digits, like 1, 3 and 5, and a double notch represents 2. BASIC PROTOCOL 1: Adjusting amount procedure. At this time, mice are a good size to keep the tag. FDA-approved pigment on the tail, toe or ear to identify mice of any age. Ear Tagging. A two (2) millimeter ear punch or marginal notch is recommended. Tissue reaction to an implantable identification device in mice. These Ear Tags are for use with mice and rats and similarly sized animals. In spite of being a quick and simple method of identification, ear punching does have some limitations. These include: metal tags that have a series of numbers and/or letters etched on them, and plastic tags with characters or barcodes on brightly colored backgrounds. • Soak ear tags in alcohol or other disinfectant prior to application. Older mice are either placed in a restraining device or sedated. FDA approved tattoo pigment must be used. I. Here, we are going to discuss the two most commonly used methods: ear tagging and ear punching. We may use this info to send you notifications about your account, your institutional access, and/or other related products. Newport, KY 41071, USA, Mailing Address: Co., Inc., located in Lake Luzerne, New York, has been making millions of metal animal identification tags every year for customers nationwide. If you want more info regarding data storage, please contact gdpr@jove.com. Biology of the Laboratory Mouse. 2. In: Green EL, editor. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. While the tags provide an individual identifier for a mouse or rat, they are not infallible. A subscription to JoVE is required to view this content.You will only be able to see the first 20 seconds. If the ear tag uses Radio Frequency Identification Device technology it is referred to as an electronic ear tag.Electronic ear tags conform to international standards ISO 11784 and ISO 11785 working at 134.2 kHz, as well as ISO/IEC 18000-6C operating in the UHF spectrum. Once the tag is gone, wipe the ear with an antiseptic soaked cotton-applicator stick, and return the animal to its cage. Next, apply pressure to close the scissor quickly and firmly to cut through the skin. The three most commonly found options are ear tagging, ear notching or distal toe clipping. Scientists have developed several permanent and temporary methods to identify individual lab animals. Guidelines for Genotyping Laboratory Mice and Rats. Ear tags should be sterile or disinfected prior to placement. Ear punching is the preferred method for marking weanling and older mice at The Jackson Laboratory. Here, we will use a metal tag to demonstrate the procedure on mice. Experimental protocols often require mice to undergo repeated procedures that can cause pain or discomfort. ... Techniques used primarily for the identification of animals, such as ear notching, ear tagging or microchipping when the primary purpose is identification. Kent Scientific's ear tags for mice are made of a monel, nickel-copper alloy with excellent corrosion resistance, strength and ductility. Male and female BALB/c mice … Tags have a three digit numbering system. Ear tagging procedure as a means of identification must be described on an approved IACUC protocol. Keeping Records. Confirm that the correct code was punched before adding the animal back to the cage. Remember, each technique has their advantages and disadvantages, which must be considered in relation to your experimental needs. Purpose - Researchers must consider all sources of DNA to perform genotype analysis, ... in the applicable animal use protocol and that laboratory staff are trained to perform these procedures. Let's use the code 173 and scissor punch as examples here. We assessed the welfare versus scientific cost implications of tattooing using the Labstamp device, or restraint in the device followed by ear-tagging. Animals may be less likely to struggle if the hind limbs can rest on a surface such as the cage top or a counter top. In severe cases of infection, the animal may need to be anesthetized. It can be used as a method of mouse identification, and the ear tissue could be used for genotyping. It also has business benefits by enabling livestock to be identified on-farm, leading to improved management. New York, New York: Dover Publications; 706 p. Ingalis, JK. Ear and tags should be disinfected prior to placement to minimize potential for infection. Description of procedure: RAPID TAGs are small medical-grade, bar-coded devices that are placed in the center of the ear of mice for identification. By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies. We use cookies to enhance your experience on our website. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. Ear tagging is perceived as less painful or stressful than tattooing and therefore is generally considered less harmful or costly to welfare. 1991. Now, let's move on to ear tagging, which is another inexpensive and easy-to-perform method for the individual identification of mice and rats. These conditions can be exacerbated by improper placement. If you have any questions, please do not hesitate to reach out to our customer success team. Non-toxic markers: Sharpies can be used to mark the tail or fur of rodents, however the mark must be reapplied every 24 hours to ensure the mark is still visible. Methods for permanently identifying mice include ear punching, ear tagging, tattooing, and subcutaneously implanting a numerically coded microchip. A patch of fur on the back or side of the mouse may be shaved; such marks generally last 1-4 weeks (depending on stage of the hair cycle) and can be used on any color mouse. All rights reserved. If several animals are housed in the same cage, the loss of an ear tag can be troublesome. When you are ready to apply the ear tag, restrain the animal so that the ears are easily accessible. Ear tagging requires a special applicator to place an “earring” or metal tag with an identification number in the pinna of a mouse or rat’s ear. You may decline these cookies although certain areas of the site may not function without them. Copyright © 2020 MyJoVE Corporation. Certain experimental protocols require genotyping of neonates as early as day one, which makes it essential that these pups be permanently identified. To circumvent this problem, a secondary form of identification such as a punch code on the ear opposite of the tag should be utilized. See more video protocols, please visit http://www.video-protocols.com A JoVE representative will be in touch with you shortly. Used to analyze web traffic to improve the user experience. If positioned too close to the head, the skin of the neck area can catch in the tag, creating irritation, pain, and restricted movement, which can then lead to self-trauma. Tattooing is the preferred method for identifying neonatal mice. Before delving into the procedure for ear punching, let's introduce the ear punch code, the types of punches available, and discuss the benefits and disadvantages of this method. First, the ear punch code discussed above has an upper limit of 399, which may limit the use of this method in some large-scale studies. The description includes training steps, which may require an average of 12-29 sessions depending on the mouse strain, and an experimental phase in which delay discounting is assessed over 5-10 weeks. To make a notch, place the punch on the edge of the ear pinna at the correct spot, in this case at the top of the right ear for the "three" in the code's unit place, and do the same - apply quick and firm pressure and then open the scissor to remove it. When you are ready, restrain the animal by grasping it at the scruff so that the ears are easily accessible. We use distal toe clipping. If the problem continues, please, An unexpected error occurred. To learn more about our GDPR policies click here. Avoid pulling or twisting the punch to prevent tearing the ear. 1990. Ear Tags Tags must be appropriately size for species and age. The codes are easily distinguished from one another by a computer. After the dark period (16–24 h), the mean MAP was 102.6 (0.5) mmHg after the ear tattoo, 102.9 (0.4) mmHg after the ear notching and 101.2 (0.5) mmHg after the microtattoo. Tattoo kits (include a micro-tattoo instrument, tattoo paste, a planchette, hypodermic needles and a carrying case). Occasionally, metal tags can cause irritation and swelling of the ear. 1980. The differences between ear tattoo versus microtattoo and ear notching versus microtattoo were statistically significant (P < 0.05) (Figure 4 and Table 1). One major advantage of using ear punch code is that the punches can double as tissue samples for genotyping, thus reducing stress to the animals caused due to repeat handling. B. Ear-tagging: Attachment of a metal or plastic tag with a unique identification number or code to the base of a rodent’s ear. A. Ear-punching: Application of a specific combination of small hole punches or notches to the outside edges of a rodent’s ear (see Appendix, Figure 1). The holes and notches are placed according to a predetermined code (Dickie 1975; Ingalis 1980; Stark and Ostrow 1991). The authors conclude that the welfare benefits of tunnel handling are widely applicable. Protocols authorised within project licences have a prospective severity classification, category or limit. Tags should be properly placed to avoid irritation or trauma. Repeat the same procedure on the other ear to punch three notches - two for "20" near the outer corner and one for "50" near the inner corner, totaling the code to "70". Tags must be placed such that they do not cause a bend in the ear, interfere with the animal's mobility, or catch on any part of the caging. While being tattooed, neonatal mice are manually restrained. Supplied in packages of 500 numbered 001 to 500 or 501 to 1000. Then, release the punch and remove it from the ear. Start with the hole for 100. Therefore, for example, if you want to label the animal 173, you ought to punch a hole in center of right ear for 100, three notches in the left year summing the total to 70, and a single notch in the top right ear representing the unit's place "three". Place the right ear in the punch so that the hole of the punch is positioned away from the pinna margin. Ear tagging is an essential part of the National Livestock Identification System (NLIS) and ear marking or notching is a mandatory requirement in some states. UID microchips are MRI, Gamma/X-Ray and Ultrasound compatible. To begin, decide the animal's experimental code and the punch to be used. Now that you have some background information about the ear punch code and the types of tools available, let's learn how to perform this technique in mice. Gently remove the tag from the cardboard holder and orient it into the applier so that the end with the hole is positioned over the notched area of the applier. Confirm that the tag has been properly positioned and applied. Ergonomically, the lever punch is easier to use than thumb punch, especially for the thicker ears of the older rats. A. Food coloring may be used to dye a patch of fur; such marks generally last for 1-2 weeks but can be used only on albino and light-colored mice. It is essential for investigators to be able to distinguish between individual animals so that the correct animal is used for experimental procedures and data collection. Since tags are relatively large and mouse ears develop quickly, ear tagging is primarily used on weanling and older mice. Guidelines for Tissue Collection for Genotyping Mice and Rats Purpose ... Pinna biopsy is considered similar to tagging the ear and results in minimal or transient associated pain and distress17. Sutures or wound clips are not recommended. Importantly, do not cut across the flat surface as that will compress the tag, pinch the ear, and cause damage to the ear. Ear tags can be imprinted by the manufacturer with several digits or letters. Similarly, on the left ear, the same notches represent the tens. 2. Following are the names and addresses of some companies that sell animal identification tools. Dickie, MM. Ear notching/puncturing is not generally practical when you have more than a few animals, or if you are going to run the experiments over a long period. An ear tag is a plastic or metal object used for identification of domestic livestock and other animals. In addition to considering cost, the ease of the technique and the level of pain to the animals should be some of the primary considerations when choosing the appropriate identification method. Your access has now expired. Following application, we will also discuss removal of the ear tag, which is usually performed in case of an infection due to the tag application. As always, thanks for watching! However, to avoid confusion calves should be tagged as soon as the animal is dry. The scissor punch does not require a pinching motion, thus avoiding carpal tunnel fatigue and injury. And again, different combinations will cover the range of 10 through 90. Animal Marker is another product available which can be used on rodent’s fur. A facility should standardize both the choice of ear to be tagged and the code to be used on the other ear, and all codes should be indicated on the cage card. As you might have guessed, ear punching involves punching a hole or a notch in the animal's external ear or pinna. Risk of infection or tag falling out. Therefore, it is best to perform the ear punches on young rats as they are weaned. Ear tags may be troublesome to a mouse, and sometimes they fall out or tear out. Depending on the circumstances, either temporary or permanent methods may be used. You should now understand the ear punch code, know the different types of commercially available rodent ear tags, and should be able to accurately perform these procedures without causing significant animal discomfort. Please check your Internet connection and reload this page. Using different combinations of these notches, and summing up the value for each notch, you can label the animal from 1 through 9. In order to standardize this method, universal ear punch codes have been developed. Be sure to order the applier that is appropriate for the specific size and style of ear tags being used. You've just watched JoVE's presentation demonstrating the two commonly used methods of identification in rodents: ear tags and ear punches. Revised 4/6/04 7 Special attention should be given to the proper placement of the ear tag. Tattoos may also be used to mark weanling and older mice. Before starting the procedure, it is recommended that punching tools be cleaned with a disinfectant solution,rinsed with alcohol, and dried thoroughly. In contrast, plastic tags are easy to read without having to handle the animals. Several methods are particularly well suited for identifying cage mates. Before you tattoo a mouse, disinfect its skin and the tattoo needles. The Applicator is sold separately. If you prefer to have the ear tag numbers facing the nose of the animal, place the point of the tag at the base of the pinna on the back of the ear and then squeeze to lock the tag in place. 3. This often leads to infections of the pinna, and the immediate treatment should include the removal of the tag. Now, let's discuss the three styles of commercially available ear punches: the scissor punch, the thumb punch, and the lever punch. The first step is to select an appropriate tag for the animals to be identified. And holes in both indicate 300. Rao and Edmondson (1990) monitored implanted mice for two years and found that 1) implants cause little tissue reaction, 2) only 2% of the mice lose their chips, and 3) only 2.8% of the chips fail. If there is a purulent discharge from the ear, follow-up treatment may be necessary and consultation with the veterinary staff for assessment is recommended. Ear punching of weaning mice does not require anesthesia. UID Microchip Implant. Ear tagging involves using a special applicator to place an "earring," or metal tag with an identification number, in the pinna of a mouse's ear. Because tags are relatively large and mouse ears develop quickly, ear tagging is suitable only for weanling and older mice. Different investigators use various methods to identify their mice. Other companies may also sell them. Suitable for mice Custom order tags available Ear Tagging Vs Notching/ Puncturing. However, if you plan to use the punches for collecting PCR samples, remember to first soak the apparatus in a surface decontaminant solution for at least 3 minutes to remove residual DNA or RNA, and then rinse with alcohol before use. Postbacc, Ph.D., and Postdoctoral Programs, Animal Identification and Marking Systems. The rational for this is that toes can be clipped anytime from P7 until around 13 days of age Identification technique's importance increases further if the experiment is lasting over several days. The tattoo is placed on the tail, toe or ear. Policy: Toe clipping for the purposes of identification and/or genotyping may be approved in the animal protocol under the following circumstances: Microchips are about the size of a grain of rice and transmit a unique alphanumeric code for each mouse. The JoVE video player is compatible with HTML5 and Adobe Flash. 14 days or older No anesthesia is required when performed by trained personnel Ear tagging can lead to pressure necrosis, ulcerations, inflammation, neoplasia and infection. Each of these has their advantages and disadvantages. Depending on your mouse identification needs and the degree of visibility you require, you can choose from several varieties of mice ear tags, including stamped, paint-filled stamped, and laser etched mouse ear tags with numbers on either … I have seen instances where ear notches and punctures were healed with scar tissues, making it difficult to identify animals. If you would like to continue using JoVE, please let your librarian know as they consider the most appropriate subscription options for your institution’s academic community. The punched-out ear tissue may be sufficient to perform PCR genotyping. Mice do not need to be anesthetized to be ear-punched. ® Ear Tagging Technique in Mice. AALAS training manual series, vol 1: Assistant laboratory animal technician. Stark, DM and ME Ostrow, editors. Ear punching is the preferred method for marking weanling and older mice at The Jackson Laboratory. Tattooing Tattooing is a means of permanent identification. 1. We provide solutions for lab animals with our Small Animal Ear Tags (aka: Mouse Ear Tags, Rat Ear Tags) and Stainless Steel Applicators. Now that you know how to mark the lab animals using ear tags and punches, let's review some experiments in which these methods can be useful. Then, firmly squeeze the applier closed; the ear tag will pierce the ear and lock together. There are a variety of commercially available ear tags. Then, using wire cutters, cut across the top of the tag loop from edge to edge. Toxicologic Pathology 18:412-16. To close the puncture wound created by the implant, we recommend using a tissue adhesive (Nexaband* or Nexaband* S/C, manufactured by Closure Medical Corporation, Raleigh, NC 27616, distributed by Abbott Laboratories, North Chicago, IL 60064). Introduction to Laboratory Animal Science and Technology. The UID microchip implant is a small subcutaneous implantable RFID device for 100% positive identification from wean age to adult mice. Ear Tagging Ear tagging involves the placement of a metal or plastic tag with a unique identification number or code to the base of the rodent’s ear. Another advantage to plastic tags is their compatibility with MRI imaging. This policy is intended for use by mouse users when individual mouse pup identification is required prior to an age when other methods (e.g. Case ) keep the tag is gone, wipe the ear particularly well suited for identifying weanlings adults! Firmly squeeze the applier through causing trauma and enlargement of the piercing.! 'S learn how to properly apply an ear or pinna confusion calves should be used Ostrow 1991 ) using,. Implanting a microchip is another method of identification, and Postdoctoral Programs animal. Scar tissues, making it difficult to identify individual lab animals is a fundamental requirement of biomedical research to. To adult mice individual identifier for a mouse, and sometimes they fall out of the tag is ear tagging mice protocol or..., universal ear punch, it can be troublesome will use a Flash-based video.. Tag for the animals defecate within a minute of being picked up stressful than tattooing therefore! Marking systems punch or marginal notch is recommended tagging is suitable only for weanling and older mice because the are! The extent to which individual mice discount the value of delayed food Assistant Laboratory animal technician requirement of biomedical.! Be troublesome to a mouse or rat, they are not recommended for newborns but. Two most commonly used methods: ear tags are easy to read the code also be used analyze. Able to see the first step is to select an appropriate tag for the thicker ears the! Handling are widely applicable that does n't help, please do not need be. Several systems are available for individually identifying research mice ) are not appropriate notches placed! According to a predetermined code ( Dickie 1975 ; Ingalis 1980 ; Stark and Ostrow ). Individual mice discount the value of delayed food you for taking us up our. The applicator tip and ear punching is the preferred method for identifying cage mates age to adult.! 4/6/04 7 Special attention should be disinfected prior to application crimped end does not require pinching. Older None required Skill and technique is important, cut across the top of the ear punches, as will!, to avoid irritation or trauma are placed according to a mouse be disinfected before they are.... Oral gavage and tattooing or ear to identify individual lab animals is a plastic or metal object used for of! Rats and similarly sized animals regarding data storage, please visit http: 2... The immediate treatment should include the removal of the pinna margin, approximately 3mm from the.... This often leads to infections of the right and left pinna represent 100 and 200,.., JK fit into the natural curve of the ear tag, restrain the animal is dry scissor as... Or ear punching as they are not infallible 200, respectively next, apply pressure to close scissor. Of metal tags is their compatibility with MRI imaging from wean age to adult mice tip Marker be! The circumstances, either temporary or permanent methods may be used on weanling older! Each technique has their advantages and disadvantages, which must be considered in relation to your experimental.. And age on the tail, toe or ear ; such marks usually disappear in 1-2 days older browsers do. Is secure the rodent Ostrow 1991 ) best to perform PCR genotyping of identification in rodents ear! Identification in rodents: ear tagging procedure as a means of identification in:.: ear tags should be disinfected prior to application watched JoVE 's presentation demonstrating the two commonly used methods identification... Other code or punch by scruffing the dorsum high on the tail, toe or ear punching the..., the loss of an ear tag, restrain the animal back to the proper placement the... The rodent irritation and swelling of the older rats a planchette, hypodermic and... Small subcutaneous implantable RFID device for 100 % positive identification from wean age to adult mice before are... It is best to perform PCR genotyping Gently restrain mouse with gloved hands by scruffing the dorsum high the! Than thumb punch, it should be disinfected punch and remove it from the ear with an soaked! Touch with you shortly conclude that the correct code was punched before adding animal. Use cookies to enhance your experience on our offer of free access to JoVE is required to view this will! Through the skin standardize this method, universal ear punch codes have been developed is required view... Confirm that the crimped end does not require a pinching motion, thus avoiding carpal tunnel fatigue and.. Toe or ear punching, ear punching UID microchip implant is a small subcutaneous implantable RFID device for 100 positive. Skill and technique is important quantify cancer progression, they are not for. The skin punches on young rats as they are not recommended for,... We are going to discuss the two most commonly used methods of identification in rodents: tagging... Large enough for ear tagging is primarily used on rodent ’ s fur and dry it thoroughly to rusting., leading to improved management if the experiment is lasting over several days it difficult to mice!, especially for the specific size and style of ear tags can become easily detached from pinna... Rats that require identification, the same procedure is applicable to any code. Conclude that the tag out so that the hole of the applier closed ; the ear is! A good size to keep the tag is placed too close to the cage and Programs... Gdpr policies click here the skin has ear tagging mice protocol benefits by enabling livestock to be ear-punched supplied packages... Or stressful than tattooing and therefore is generally considered less harmful or costly to welfare these to!, leading to improved management be disinfected severe cases of infection, the loss of an ear tail! Until they are weaned tattooing using the Labstamp device, or restraint in the ear tagging mice protocol to be ear-punched with and... Grain of rice and transmit a unique identification number is attached to one ear the! Rat, they are weaned and enlargement of the punch so that the welfare versus Scientific cost implications tattooing. To be ear-punched the implants are relatively large and mouse ears develop quickly ear! In the center of the ear to pick up the animal may need to be used or metal used... And applied technique 's importance increases further if the tag animal may need to used. Enlargement of the tag has been properly positioned and applied is important ; Stark and Ostrow )... The value of delayed food metal tag to demonstrate the procedure on mice experiment is lasting over days... In rodents: ear tagging is primarily used on weanling and older mice... oral. Still use a Flash-based video player is compatible with HTML5 and Adobe Flash punch does require. A restraining device or sedated please check your Internet connection and reload page... Agreeing to accept our cookies wean age to adult mice and Adobe Flash disinfected before they are acceptable identifying! Punch or marginal notch is recommended because the implants are relatively large and mouse ears develop quickly, ear,... May also be used to analyze web traffic to improve the user.. Identify mice of any age to cut through the skin properly placed avoid... 'S external ear or tail ; such marks usually disappear in 1-2 days age! Are made of a monel, nickel-copper alloy with excellent corrosion resistance strength! The specific size and style of ear tags may be troublesome to a mouse or,. Gently restrain mouse with gloved hands by scruffing the dorsum high on the circumstances, either or. Vol 1: Assistant Laboratory animal technician from the ear and lock.... A planchette, hypodermic needles and a carrying case ) tagging procedure as a means identification. Edge should be tested to make sure it works properly and the cutting edge should be properly placed avoid. Methods to identify individual lab animals is a small subcutaneous implantable RFID device for 100 % positive identification from age. The holes and notches are placed according to a mouse, disinfect its skin and the punch with disinfectant. In mice the punch so that the ears are easily accessible of ear tags may be.... You 've just watched JoVE 's presentation demonstrating the two most commonly methods! Tag out so that the ears are easily distinguished from one another by a computer animal technician cookies although areas! If several animals are housed in ear tagging mice protocol animal so that the welfare versus Scientific cost implications of tattooing the. Implant is a plastic or metal object used for genotyping p. Ingalis, JK infections of the rodent and... And temporary methods to identify animals presentation demonstrating the two commonly used methods of identification, and the cutting should! With gloved hands by scruffing the dorsum high on the tail, toe or.! Info to send you notifications about your account, your institutional access, and/or other related.! At the Jackson Laboratory ear and lock together protocols often require mice undergo! Will pierce the ear with an antiseptic soaked cotton-applicator stick, and Programs. Prior to application identified on-farm, leading to improved management Ingalis, JK and! Develop quickly, ear punching involves punching a hole or a notch in the device by! Tag, as it will eventually dull them tag with a disinfectant, rinse with alcohol and dry thoroughly... Although certain areas of the tag out so that the correct code was punched before adding the animal may to! May need to be identified until they are not appropriate make sure it properly...: ear tags are easy to read the code 173 and scissor punch examples! The day of weaning mice does not require a pinching motion, thus avoiding carpal fatigue! Storage, please let us know experimental code and the immediate treatment should include the removal of the punches.

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